DNA+presentations-+rebecca

-purines (double ringed structures), and pyrimidines (single ringed)
 * DNA is made up of 4 different parts called nucleotides
 * made of up sugars
 * there are 4 nitrogenous
 * the A and the t match up and the G and the C match up

PCR what is it used for?- mapping technique for human genome project, DNA fingerprinting, diagnosis of genetic disorders, detection of bacteria or viruses - you need nucelioustides in the test tube, - you put a loading buffer in it
 * initialization- heats to 94-96 degrees, seperates into single strands hydrogen bonds are broken.
 * Denaturation
 * Annealing- temperatures lower to 50-65 degrees Celsius,
 * Extension/elongation - taq

Electrophoresis -is the motion of dispersed particles relative to a fluid under the influence of an electric field that is space uniform. -particles disperse in a fluid carry an electric surface - gel electrophoresis is used to separate DNA fragments -start out with a plastic tube with clear liquid in it. - DNA strands are cut and they need to be sorted/separate - YOU NEED TO MAKE MULTIPLE COPIES OF DNA AND THIS IS THE PURPOSE ELECTROPHORESIS. - you need a buffer (ph), a gel mold, - -